Gastrointestinal Cancer-associated Antigen in Immunoperoxidase Assay1
نویسندگان
چکیده
A monosialoganglioside antigen of gastrointestinal adenocarcinomas defined by murine monoclonal antibody was dem onstrated by immunoperoxidase (IP) assay in fixed paraffinembedded tumors in 59% of colonie adenocarcinomas, 86% of pancreatic adenocarcinomas, and 89% of all gastric ade nocarcinomas. In all patients with detectable levels of antigen in circulation, the resected tumors also expressed the antigen in IP assay. Six of eight individuals with no detectable levels of antigen in their serum samples expressed the antigen in the tumor tissue. Removal of the sialic acid residue of the antigen abolished the IP reaction. The successful use of the IP assay on fixed tissue to demonstrate the specific sites of gastrointes tinal cancer antigen localization in human tumors and normal tissues provides an important tool for the study of developing neoplasia.
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